Anna Barnard

Data set for: Long-Lived Azobispyrazole Stapled Peptides for Light-Activated Inhibition of Protein-Protein Interactions

Anna Barnard

Raw data associated with this publication: peptide characterisation by HPLC and mass spectrometry, circular dichroism spectroscopy, fluorescence anisotropy and photoswitch charcterisation (UV-Vis spectroscopy)

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Long-Lived Azobispyrazole Stapled Peptides for Light-Activated Inhibition of Protein-Protein Interactions

Anna Barnard

Raw data associated with this publication: peptide characterisation by HPLC and mass spectrometry, circular dichroism spectroscopy, fluorescence anisotropy and photoswitch charcterisation (UV-Vis spectroscopy)

gb (code pays fourni par la source)

Long-Lived Azobispyrazole Stapled Peptides for Light-Activated Inhibition of Protein-Protein Interactions

Anna Barnard

Raw data associated with this publication: peptide characterisation by HPLC and mass spectrometry, circular dichroism spectroscopy, fluorescence anisotropy and photoswitch charcterisation (UV-Vis spectroscopy)

gb (code pays fourni par la source)

Correlating Structure and Rheology in Ionically Crosslinked Alginate Biopolymer Hydrogels - A Case for Why “Less” can be “More”

Vinay Kopnar, Peter S. Sherin, Sarah Graham, Harriet Fyfe et autres

We probe the structural and rheological properties of ionically crosslinked alginate, a model biopolymer hydrogel, using microscopy, rheology and viscosity dependent molecular probes. This combination of techniques enables the quantification and correlation of microstructure, microviscosity, bulk viscoelasticity and yielding dynamics. By adjusting …

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Proteome-wide target profiling of a-helix mimetics

Amrita Date, Archie Wall, Hannah Kiely‐Collins, Theo Flack et autres

The dysregulation of protein–protein interactions (PPIs) in disease states is well established, yet they are challenging to target, owing to the large surface area and featureless nature of protein binding interfaces. For targeting helix-mediated interactions, α-helix mimetics present a promising strategy. These …

Author response for "Proteome-wide target profiling of α-helix mimetics"

Amrita Date, Archie Wall, Hannah Kiely-Collins, Theodore Flack et autres

Optimized Sample Handling Minimizes Peptide Adsorption to Plastics to Enable High Sensitivity Evosep Based Chemical Proteomics

Amrita Date, Anne Schuhmacher, Jianan Lü, Daria Berezina et autres

Chemical proteomics approaches often yield low peptide recovery because they aim to enrich low-abundance proteins. Poor sample handling further reduces recovery through peptide adsorption to plastic surfaces and losses due to vacuum evaporation. We systematically mapped these losses across buffers, volumes, plastics …

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Author response for "Proteome-wide target profiling of α-helix mimetics"

Amrita Date, Archie Wall, Hannah Kiely-Collins, Theodore Flack et autres

Proteome-wide target profiling of α-helix mimetics

Amrita Date, Archie Wall, Hannah Kiely‐Collins, Theodore Flack et autres

-substituted oligobenzamides, pyrrolopyrimidines, and oxopiperazines. This represents the first direct cross-comparison of different helix mimetic scaffolds, revealing significant differences in proteome-wide selectivity.

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Geminin inhibits DNA replication licensing by sterically blocking CDT1-MCM2 interactions

J. P. Tomkins, Lucy V. Edwardes, Sarah V. Faull, Matthew Peach et autres

DNA replication is tightly regulated to occur once per cell cycle, with the MCM2-7 helicase loaded onto replication origins only during G1-phase. In higher eukaryotes, geminin negatively regulates this process during S-, G2- and M-phases by binding the essential licensing factor CDT1. …

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Author response for "Affinity-based protein profiling of MDM2 inhibitor Navtemadlin"

Amrita Date, Archie Wall, Peiyu Zhang, Jack W. Houghton et autres

Affinity-based protein profiling of MDM2 inhibitor Navtemadlin

Amrita Date, Archie Wall, Peiyu Zhang, Jack W. Houghton et autres

target discovery for Navtemadlin through affinity-based protein profiling. MDM2 was robustly identified as the main target, across two cell lines, using two distinct probe designs. While off-targets were identified, these were not consistent across cell lines and probe designs, consistent with a …

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