Katayoun Heshmatzad

Protocol for enhancing CRISPR-Cas9 genome editing using histone deacetylase inhibition and engineered virus-like particle delivery

Mahbod Djamshidi, Ryota Tanida, Katayoun Heshmatzad, Hokan Krowicki et autres

We present a 10-fold faster, accurate, and more efficient (FAME)-CRISPR-Cas9 gene editing workflow utilizing histone deacetylase inhibitor (HDACi)-mediated chromatin relaxation and engineered virus-like particle (eVLP) delivery of Cas9. We describe steps for optimizing HDACi concentration, euchromatinization timing, and Cas9 delivery/expression to improve …

ca (code pays fourni par la source)

Natural bioactive molecules and chemotherapeutics synergism for enhanced cancer therapy

Mehrdad Hashemi, Katayoun Heshmatzad, Ghazaleh Shahsavan, Vahid Tavakolpour et autres

BACKGROUND: Chemotherapy remains a foundation of cancer care but is limited by multidrug resistance, systemic toxicities, and suboptimal selectivity, prompting interest in adjunctive strategies that improve efficacy and tolerability without adding significant burden to patients or healthcare systems. AIMS/OBJECTIVES: This review highlights …

ir, ca, cn (code pays fourni par la source)

FAME-CRISPR improves CRISPR-Cas9 genome editing via HDAC inhibition and engineered virus-like particle delivery

Mahbod Djamshidi, Alexander Hill, Katayoun Heshmatzad, Jethro Langley et autres

CRISPR-mediated gene editing using engineered virus-like particles (eVLPs) can achieve high efficiency, but performance varies with reduced effectiveness often seen in primary cells or when generating polyclonal models at scale. We developed a faster, accurate and 4-fold more efficient CRISPR-Cas9 (FAME-CRISPR) method …

ca (code pays fourni par la source)

Genetic Variations in the Human Angiotensin-ConvertingEnzyme 2 and Susceptibility to Coronavirus Disease-19

Taravat Talebi, Tannaz Masoumi, Katayoun Heshmatzad, Mahshid Hesami et autres

Background. Health and economies are both affected by the coronavirus disease-19 (COVID-19) global pandemic. Angiotensin-converting enzyme 2 (ACE2) is a polymorphic enzyme that is a part of the renin-angiotensin system, and it plays a crucial role in viral entry. Previous investigations and …

ir (code pays fourni par la source)

Polymorphism of rs599839 in the PSRC1 gene is associated with coronary artery disease in an Iranian population

Golnaz Houshmand, Mohammad Javad Alemzadeh‐Ansari, Saeideh Mazloumzadeh, Niloofar Naderi et autres

Introduction: Coronary artery disease (CAD) is the leading health complication worldwide because of its high prevalence and mortality. The association between CAD susceptibility and the rs599839 (C/T) polymorphism in the human proline and serine-rich coiled-coil (PSRC1) was reported in a genome-wide association …

ir (code pays fourni par la source)

Role of non‐coding variants in cardiovascular disease

Katayoun Heshmatzad, Niloofar Naderi, Majid Maleki, Shiva Abbasi et autres

Cardiovascular diseases (CVDs) constitute one of the significant causes of death worldwide. Different pathological states are linked to CVDs, which despite interventions and treatments, still have poor prognoses. The genetic component, as a beneficial tool in the risk stratification of CVD development, …

ir (code pays fourni par la source)

Designing and Development of a DNA Vaccine Expressing HER2/ HER3 and CCL20 as a Novel Candidate for Breast Cancer Vaccine

Katayoun Heshmatzad, Amir Gholamzad, Mehrdad Gholamzad, Maryam Eslami

Abstract Background: Breast cancer (BC) is one of the most common diseases among women worldwide and about 2.1 million new cases were diagnosed in 2018. Despite effective treatments such as chemotherapy, radiotherapy and Hormone therapy, BC has been remained as one of …

ir (code pays fourni par la source)

Identification of a novel de novo pathogenic variant in GFAP in an Iranian family with Alexander disease by whole-exome sequencing

Katayoun Heshmatzad, Niloofar Naderi, Tannaz Masoumi, Hamidreza Pouraliakbar et autres

BACKGROUND: Alexander disease (AxD) is a rare leukodystrophy with an autosomal dominant inheritance mode. Variants in GFAP lead to this disorder and it is classified into three distinguishable subgroups: infantile, juvenile, and adult-onset types. OBJECTIVE: The aim of this study is to …

ir (code pays fourni par la source)

GFAP variants leading to infantile Alexander disease: Phenotype and genotype analysis of 135 cases and report of a de novo variant

Katayoun Heshmatzad, Mahya Haghi Panah, Ali Reza Tavasoli, Mahmoud Reza Ashrafi et autres

ir (code pays fourni par la source)

Effects of morphine and NeuroAid on the expression levels of GluN2A and GluN3A in the hippocampus and striatum of rats.

Katayoun Heshmatzad, Mohammad Nasehi, Salar Vaseghi

OBJECTIVES: NMDA glutamatergic receptors are heteromeric receptors with various subunits. GluN2A and GluN3A subunits specify the functional heterogeneity of NMDA receptors. These subunits play a key role in the induction of LTP and synaptic plasticity. Note that, the function of NMDA subunits …

ir (code pays fourni par la source)

The Genetic Perspective of Familial Glucocorticoid Deficiency: In Silico Analysis of Two Novel Variants

Katayoun Heshmatzad, Nejat Mahdieh, Ali Rabbani, Abdollah Didban et autres

Familial glucocorticoid deficiency is a rare autosomal recessive genetic disorder which belongs to a group of primary adrenal insufficiency (PAI) and is mainly caused by mutations in the MC2R and MRAP genes. A comprehensive search was conducted to find the reported variants …

ir (code pays fourni par la source)

PPARG (Pro12Ala) genetic variant and risk of T2DM: a systematic review and meta-analysis

Negar Sarhangi, Farshad Sharifi, Leila Hashemian, Maryam Hassani Doabsari et autres

Abstract Type 2 diabetes mellitus (T2DM) is a complex disease caused by the interaction between genetic and environmental factors. A growing number of evidence suggests that the peroxisome proliferator-activated receptor gamma (PPARG) gene plays a major role in T2DM development. Meta-analysis of …

ir (code pays fourni par la source)