D4:'Paper on the applicability and limitations of methods to determine the lipid composition and structural integrity of lipid - based NPs submitted to a peer - reviewed journal.'
Rattachement africain : fr, se, gb, it, de, us, no, ch. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Accepted version of deliverable D4 Liposomes and lipid nanoparticles (LNPs) are central to modern drug‑delivery strategies, yet their reliable characterisation remains challenging due to their structural complexity and the limited availability of harmonised analytical standards. In this study, we assess an extensive set of established and novel dimensional, structural, and chemical characterisation methods across a panel of well‑defined LNP formulations and two liposomal systems with distinct compositions and stabilisation mechanisms. Cryogenic transmission electron microscopy provided direct visualisation of particle morphology and lamellarity, revealing clear structural differences between homogeneous, predominantly unilamellar liposomes and heterogeneous, multilamellar liposomes, and confirming the structural consistency of LNP samples. Dimensional methods for particles in fluids generated complementary size metrics and highlighted differences in resolution as well as method‑dependent artefacts, particularly for polydisperse samples. Among these, small angle X-ray scattering unlocked structural information including bilayer thickness and multilamellar spacing under native liquid conditions, while ribonucleic acid (RNA)-specific assays provided robust quantification of total and encapsulated RNA. Advanced electron- and mass‑spectrometric techniques, delivered additional insight into surface chemistry and, critically, enabled molecular‑level analysis at the single‑particle scale. Together, these results show that no single technique can fully capture the complexity of lipid‑based delivery systems. Instead, a metrologically informed, multimodal approach is essential for generating reliable, reproducible datasets and for supporting the development of future standards for the characterisation and quality control of nanomedicine formulations. This study was funded by the 22HLT04 MetrINo project and comprises deliverable D4. The project has received funding from the European Partnership on Metrology, co-financed from the European Union’s Horizon Europe Research and Innovation Programme and by the Participating States.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
Où se fait cette recherche
-
Laboratoire National de Métrologie et d'Essais pays non établi dans la noticeInstitution
-
RISE Research Institutes of Sweden pays non établi dans la noticeInstitution
-
National Physical Laboratory pays non établi dans la noticeStructure de recherche
-
University of Pavia pays non établi dans la noticeUniversité ou école supérieure
-
Université de Pau et des Pays de l'Adour pays non établi dans la noticeUniversité ou école supérieure
-
Physikalisch-Technische Bundesanstalt pays non établi dans la noticeOrganisme public
-
Analytical Services pays non établi dans la noticeOrganisation à but non lucratif
-
Nanobiotix (France) pays non établi dans la noticeEntreprise
-
SINTEF pays non établi dans la noticeStructure de recherche
-
SINTEF Industry pays non établi dans la noticeStructure de recherche
-
Swiss Federal Laboratories for Materials Science and Technology pays non établi dans la noticeStructure de recherche
-
Kratos Analytical Ltd pays non établi dans la noticeEntreprise
Laboratoire National de Métrologie et d'Essais, RISE Research Institutes of Sweden et National Physical Laboratory, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.