Molecular detection and characterization of a ‘Candidatus Phytoplasma’ strain infecting tomato crops in Assam, India

Le résumé fourni par la source

Tomato (Solanum lycopersicum), one of the most important vegetables consumed globally is also a major crop grown in the state of Assam, India. It is reported to be infected by phytoplasmas from various other states of India. Field surveys were conducted during 2025-2026 across the major tomato growing regions of Assam and different phytoplasma associated symptoms like, little leaf, witches’ broom, malformed flower and leaf, curling and crinkling and chlorosis were observed. Symptomatic tomato plants were tested for presence of phytoplasmas using PCR with P1/P6 primers that produced an about 1.5 kb DNA band and nested PCR with R16F2n/R16R2 primers that gave an about 1.25 kb band in agarose gel. Sequencing and phylogenetic analysis of these bands showed the higher sequence similarities with strain of ‘Candidatus Phytoplasma trifolii’. Furthermore, virtual restriction fragment length polymorphism analyses showed the closer relatedness of this phytoplasma strain from Assam with strains in ribosomal subgroup 16SrVI-D

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