ALA-PDT Induces Ferroptosis in HPV-Infected Cells and Promotes M1 Macrophage Polarization
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Le résumé fourni par la source
Condyloma acuminatum (CA) is a recurrent human papillomavirus (HPV)-associated lesion characterized by a locally immunosuppressive microenvironment. Although 5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) has been used as a local treatment option for CA and other HPV-related lesions, the mechanisms underlying its immunomodulatory effects remain incompletely understood. In this study, we investigated whether ALA-PDT-induced ferroptosis is associated with macrophage polarization and immune microenvironment-related changes in experimental CA models. HPV16-positive SiHa cells were used as an HPV-positive epithelial cell model to examine the cellular response to ALA-PDT, and the findings were further validated in an ex vivo CA tissue culture model. ALA-PDT significantly reduced the long-term clonogenic growth of SiHa cells in vitro, as demonstrated by colony formation assays. Mechanistically, ALA-PDT suppressed the GPX4/SLC7A11 antioxidant system and increased the accumulation of reactive oxygen species, malondialdehyde, and lipid peroxides in SiHa cells, indicating ferroptosis-associated cellular changes. In a Transwell co-culture system, ALA-PDT-treated SiHa cells promoted macrophage polarization toward an M1-like phenotype, as indicated by increased expression of CD86, iNOS, and IL-1β and decreased expression of CD206 and CD163. These effects were partially attenuated by ferrostatin-1. Consistently, ALA‑PDT reduced GPX4 and SLC7A11 expression in ex vivo condyloma acuminatum tissues, concomitant with an increase in CD86‑positive macrophages and a decrease in CD206‑positive macrophages. Collectively, these findings suggest that ALA-PDT suppress clonogenic growth, induce ferroptosis-associated changes, and modulate macrophage polarization in experimental CA-related models. Further studies are required to determine whether these effects contribute to lesion clearance and therapeutic responses in vivo.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- ALA-PDT induces ferroptosis in HPV-infected cells and promotes M1 macrophage polarization
- Date Crossref
- 01/10/2026
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Zigong First People's Hospital pays non établi dans la noticeÉtablissement de santé
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Southern Medical University Department of Dermatology pays non établi dans la noticeUniversité ou école supérieure
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Fujian Medical University Fuzhou Dermatology Institute pays non établi dans la noticeUniversité ou école supérieure
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Fuzhou General Hospital of Nanjing Military Command pays non établi dans la noticeÉtablissement de santé
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First Affiliated Hospital of Fujian Medical University pays non établi dans la noticeÉtablissement de santé
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Meizhou City People's Hospital pays non établi dans la noticeÉtablissement de santé
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Nanfang Hospital pays non établi dans la noticeÉtablissement de santé
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Dongguan People’s Hospital pays non établi dans la noticeÉtablissement de santé
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Meizhou People's Hospital Department of Plastic Surgery pays non établi dans la noticeÉtablissement de santé
Zigong First People's Hospital, Department of Dermatology — Southern Medical University et Fuzhou Dermatology Institute — Fujian Medical University, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.