Genome-guided transcriptome assembly and predicted proteome of Cristatella mucedo (Bryozoa)

Le résumé fourni par la source

Genome-guided transcriptome assembly of Cristatella mucedo polypides was generated from publicly available stage-specific SRA data (Kvach et al., 2025) and the Hi-C genome assembly of Hoencamp et al. (2021). Raw reads were quality- and adapter-trimmed, filtered for rRNA (SortMeRNA + SILVA), and mapped with STAR. Transcripts were assembled and merged with StringTie. Sequences were extracted with gffread. Open reading frames were predicted using TD2. Files included: Transcriptome assembly (FASTA) Predicted protein sequences (FASTA) References Hoencamp, C., Dudchenko, O., Elbatsh, A. M. O., Brahmachari, S., Raaijmakers, J. A., van Schaik, T., Sedeño Cacciatore, Á., Contessoto, V. G., van Heesbeen, R. G. H. P., van den Broek, B., Mhaskar, A. N., Teunissen, H., St Hilaire, B. G., Weisz, D., Omer, A. D., Pham, M., Colaric, Z., Yang, Z., Rao, S. S. P., Mitra, N., Lui, C., Yao, W., Khan, R., Moroz, L. L., Kohn, A., St. Leger, J., Mena, A., Holcroft, K., Gambetta, M. C., Lim, F., Farley, E., Stein, N., Haddad, A., Chauss, D., Mutlu, A. S., Wang, M. C., Young, N. D., Hildebrandt, E., Cheng, H. H., Knight, C. J., Burnham, T. L. U., Hovel, K. A., Beel, A. J., Mattei, P.-J., Kornberg, R. D., Warren, W. C., Cary, G., Gómez-Skarmeta, J. L., Hinman, V., Lindblad-Toh, K., Di Palma, F., Maeshima, K., Multani, A. S., Pathak, S., Nel-Themaat, L., Behringer, R. R., Kaur, P., Medema, R. H., van Steensel, B., de Wit, E., Onuchic, J. N., Di Pierro, M., Lieberman Aiden, E., and Rowland, B. D. 2021. 3D genomics across the tree of life reveals condensin II as a determinant of architecture type. Science 372:984−989. https://doi.org/10.1126/science.abe2218 Kvach, A. Y., Kutyumov, V. A., Starunov, V. V., and Ostrovsky, A. N. 2025. Transcriptomic landscape of polypide development in the freshwater bryozoan Cristatella mucedo: From budding to degeneration. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 344:119−135. https://doi.org/10.1002/jez.b.23285

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.