Synthesis of biogenic silver nanoparticle-loaded porous chitosan/polycaprolactone scaffold for in vitro wound healing assessment

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Polymer nanocomposites have garnered global attention for their significant potential as biomaterials for wound dressings. The present study aims to investigate the in vitro wound-healing potency of a biogenic silver nanoparticle-loaded chitosan/polycaprolactone (CS/PCL)- based scaffold by the scratch assay method. The silver nanoparticles (AgNPs) were synthesised from a medicinal plant, Hemigraphis alternata ( Murikootti ), via a green method. These nanoparticles were blended with the CS/PCL and used to fabricate a scaffold by the freeze-drying method. The characterisation of AgNPs and the fabricated scaffolds were carried out with the help of UV- Visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD), Dynamic light scattering (DLS), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM), Energy-dispersive X-ray spectroscopy (EDS) and Transmission electron microscopy (TEM), which confirmed the formation of AgNPs and uniform distribution of AgNPs in the scaffolds. A comparative study of physicochemical properties was carried out among the fabricated scaffolds. The agar well diffusion method and time-kill assays were performed against both Gram-positive and Gram-negative bacteria. The cytocompatibility was explored using the MTT assay in L929 mouse fibroblast cell lines. CS-PCL-Ag2 (0.05w/v%) exhibited desirable tensile strength, WVTR, and porosity. Agar diffusion assays demonstrated formulation- and organism-dependent antibacterial activity, and it was non-toxic to fibroblast cells. The scratch assay revealed that CS-PCL-Ag2 (0.05w/v%) has substantial in vitro wound-healing potency and therefore represents a promising candidate for further evaluation as a wound-dressing material.

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