HOE Legacy 2B Leucine Incorporation

Résumé fourni par la source

Rates of heterotrophic bacterial production were estimated based on the incorporation of 3H-leucine into protein. Water samples were collected during the SCOPE Hawaii Ocean Experiment - Legacy 2B (HOE Legacy 2B) cruise in July and August 2015 at Station ALOHA. For these incubations, seawater was subsampled from the CTD rosette into quaduplicate 25 mL polycarbonate centrifuge tubes from each depth. Each tube was amended with 20 nmol L-1 3H-leucine. One 25 mL tube per depth was processed immediately as a time zero blank. The remaining triplicate samples were incubated in the dark on a free-floating in situ array. To terminate the incubations, triplicate aliquots from each sample tube were added to 2 mL microcentrifuge tubes containing 100 ul of 100% trichloroacetic acid (TCA). In the shore-based laboratory, samples were processed following the microcentrifuge method.

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