Profiling Fecal Microbiome and Metabolites in a Feline Infectious Peritonitis Virus Challenge Model in Kittens: A Pilot Discovery Study
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Le résumé fourni par la source
Feline infectious peritonitis (FIP), caused by FIPV, is a highly fatal disease. The coordinated shifts in gut microbiota, metabolome, and their interactions following FIPV infection remain poorly understood. In this pilot discovery work, we established an oral FIPV DF-2 challenge model in kittens, which reproduced typical FIP phenotypes confirmed by clinical scores and pathology for exploratory multi-omics profiling. Using V3–V4 16S rRNA sequencing on Illumina PE300 and UHPLC-MS-based untargeted metabolomics, combined with LEfSe, OPLS-DA, and Pearson correlation, we preliminarily characterized fecal microbial and metabolic alterations associated with FIP onset in this exploratory animal model. FIP-affected kittens exhibited reduced abundances of Ligilactobacillus, Bifidobacterium, and Limosilactobacillus, alongside elevated Roseburia, Helicobacter, and Campylobacter. Differential metabolites were enriched in six core pathways, which preliminarily suggest potential links to oxidative stress, energy metabolism impairment, immune suppression, and systemic inflammatory response during FIP progression. Correlation analyses linked beneficial genera to upregulated 4-hydroxynonenal and xanthosine, while opportunistic taxa (Helicobacter, Campylobacter, Enterobacterales) correlated with caffeine, 1-methyluric acid, and retinoic acid derivatives. These preliminary findings suggest unique fecal microbial-metabolic signatures in FIP, providing hypothesis-generating mechanistic insights and potential therapeutic targets. However, given the exploratory nature of this pilot study, these results require validation in larger, independently controlled cohorts.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Profiling Fecal Microbiome and Metabolites in a Feline Infectious Peritonitis Virus Challenge Model in Kittens: A Pilot Discovery Study
- Date Crossref
- 02/09/2026
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Ludong University pays non établi dans la noticeUniversité ou école supérieure
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Shandong Agricultural University pays non établi dans la noticeUniversité ou école supérieure
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Collaborative Innovation Center for the Pet Infectious Diseases and Public Health in the Middle and Lower Stream Regions of the Yellow River pays non établi dans la noticeInstitution
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College of Veterinary Medicine pays non établi dans la noticeUniversité ou école supérieure
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Provincial Engineering Research Center for Pet Animal Vaccines pays non établi dans la noticeStructure de recherche
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School of Life Sciences pays non établi dans la noticeUniversité ou école supérieure
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Yantai Key Laboratory of Animal Pathogenetic Microbiology and Immunology pays non établi dans la noticeStructure de recherche
Ludong University, Shandong Agricultural University et Collaborative Innovation Center for the Pet Infectious Diseases and Public Health in the Middle and Lower Stream Regions of the Yellow River, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.