Non-adhesive functions of E-cadherin promote cancer cell survival and metastasis

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Dataset includes seven plasmid sequences (.fasta) used for generation of E-cadherin point mutations and truncations used to interrogate divergence of extracellular and intracellular domains in the context of adhesion, ROS metabolism, and metastasis. The dataset includes the XPIR expression vector and a series of engineered mouse E-cadherin constructs, including full-length E-cadherin (Cdh1-FL) and variants containing targeted amino-acid substitutions or domain deletions designed to disrupt extracellular adhesion, intracellular protein interactions, or specific regions of the cytoplasmic domain. These plasmids were used for structure–function rescue experiments in E-cadherin-deficient MMTV-PyMT tumor cells to determine which regions of E-cadherin are required to suppress mitochondrial ROS and support three-dimensional colony formation and metastatic survival. The dataset provides the plasmid identities and associated construct information needed to reproduce or extend these experiments. Collectively, the constructs enable investigation of E-cadherin functions that are mechanistically separable from canonical cell–cell adhesion and can be reused for studies of E-cadherin domain function, oxidative stress regulation, epithelial tumor biology, and metastasis. The plasmids contain recombinant DNA intended for research use and should be handled in accordance with applicable institutional biosafety requirements. No human participant data or personally identifiable information are included in this dataset.

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