Dual Traditional Chinese Medicine-Preconditioned Stem Cell Secretomes in Coaxial Electrospun Nanofibers Synergistically Accelerate Diabetic Wound Regeneration
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Le résumé fourni par la source
Abstract Chronic diabetic wounds resist healing due to impaired angiogenesis, stalled cellular migration, and persistent inflammation, a challenge further compounded by the rapid degradation of therapeutic growth factors in the proteolytic wound bed. To overcome this, Traditional Chinese Medicine (TCM) compounds are employed not as standalone drugs, but as biomolecular stimuli to precondition the secretome of Wharton’s Jelly-derived mesenchymal stem cells (WJMSCs). To overcome these critical translational barriers, this study engineers a core-shell coaxial electrospun nanofibrous scaffold (polyvinyl alcohol core/gelatin shell) designed for the stabilizing and sustained dual-delivery of biologics. We introduce a novel synergistic payload with WJMSCs conditioned medium (WJMSCs-CM) uniquely primed by two specific chinese herbal compounds, Astragaloside IV (AS-IV) and Formononetin (FMN). This core-shell architecture provides native-like contact guidance for cells while converting conventional burst release into a sustained, weeks-long elution. In vitro , this functionalized scaffold restores Akt/eNOS signaling, rescues cellular viability, and promotes robust tube formation in high-glucose-stressed fibroblasts and endothelial cells. In vivo , within an STZ-induced diabetic rat model, the application of this WJMSCs-CM-loaded coaxial scaffold actively inhibits early inflammation and comprehensively accelerates healing, driving near-complete wound closure (98.2 ± 1.5% by day 21), mature collagen deposition, and hair follicle neogenesis. Ultimately, this bio-instructive platform successfully integrates physical structural cues with sustained biochemical signaling, offering a potent, multifaceted strategy for chronic wound regeneration. Graphical abstract (Figure created in BioRender. Huang, L. C. (2026))
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Dual Traditional Chinese Medicine-Preconditioned Stem Cell Secretomes in Coaxial Electrospun Nanofibers Synergistically Accelerate Diabetic Wound Regeneration
- Date Crossref
- 28/08/2026
- Éditeur
- openRxiv
- Type
- posted-content
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Fraunhofer Institute for Molecular Biology and Applied Ecology pays non établi dans la noticeStructure de recherche
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China Medical University Graduate Institute of Biomedical Sciences pays non établi dans la noticeUniversité ou école supérieure
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Taipei Medical University Hospital pays non établi dans la noticeÉtablissement de santé
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China Medical University Hospital pays non établi dans la noticeÉtablissement de santé
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Asia University Department of Bioinformatics and Medical Engineering pays non établi dans la noticeUniversité ou école supérieure
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School of Post-Baccalaureate Chinese Medicine pays non établi dans la noticeUniversité ou école supérieure
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School of Chinese Medicine pays non établi dans la noticeUniversité ou école supérieure
Fraunhofer Institute for Molecular Biology and Applied Ecology, Graduate Institute of Biomedical Sciences — China Medical University et Taipei Medical University Hospital, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.