Deterministic optical redox (bio)sensing with molecular switch quantum defects

Le résumé fourni par la source

Fluorescent sensors are powerful tools in the life sciences, but they lack the discrete relation between analyte concentration and signal intensity known from electrochemical sensors. Here, we present a fluorescent material equipped with a molecular redox switch that enables quantitative optical redox sensing. We incorporate ferrocene (Fc) quantum defects into the lattice of fluorescent single-walled carbon nanotubes (SWCNTs). Switching the Fc2+/Fc3+ redox states either with analytes or electrochemically modulates the defect-specific near infrared emission at 1130 nm by up to 300%. These sensors directly detect redox-active molecules such as nitric oxide (NO) and hydrogen peroxide (H2O2) in the µM range. Coupling enzymatic oxidases further enables optical detection of non-redox active biomolecules. Finally, we deploy these sensors in vivo to reveal dynamic (NO) stress signalling in living plants. This architecture provides a generalizable framework for quantitative optical redox sensing.

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