Alkali-Induced ZIF-8 for Enhanced Lipase Immobilization and Enantioselective Resolution of (R,S)-1-Phenylethanol
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Le résumé fourni par la source
Zeolitic imidazolate framework-8 (ZIF-8) is an important support for enzyme immobilization due to its good biocompatibility and structural tunability. However, the inherent structural characteristics of conventional ZIF-8 suffer from limited specific surface area, thereby restricting the achievable enzyme loading. In this study, we developed a novel alkali-induced (IA) strategy using sodium hydroxide to prepare IA-ZIF-8 with markedly enhanced textural properties. Subsequently, Pseudomonas fluorescens lipase (PFL) was successfully immobilized onto IA-ZIF-8 via physical adsorption, and the optimal conditions were systematically investigated. Compared with conventional ZIF-8, IA-ZIF-8 achieved a larger specific surface area (1462.3 m2/g), and a 9.8% increase in PFL loading. The resulting biocatalyst, IA-ZIF-8-PFL, exhibited significantly enhanced thermal and pH stability due to the confinement effect and protective microenvironment from the carrier. When the biocatalyst was applied to the chiral resolution of (R,S)-1-phenylethanol, it showed satisfactory catalytic activity and enantioselectivity with a conversion of 24.4% and an ees of 25.7%. In addition, IA-ZIF-8-PFL retained satisfactory reusability over five successive cycles. This work demonstrates that IA-ZIF-8 is a highly efficient carrier for immobilizing lipase and offers a green biocatalyst for the efficient preparation of chiral drug intermediates.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Alkali-Induced ZIF-8 for Enhanced Lipase Immobilization and Enantioselective Resolution of (R,S)-1-Phenylethanol
- Date Crossref
- 25/08/2026
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Suzhou Chien-Shiung Institute of Technology Suzhou Chien-Shiung Biopharmaceutical Industry Think Tank pays non établi dans la noticeStructure de recherche
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Huazhong University of Science and Technology pays non établi dans la noticeUniversité ou école supérieure
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College of Biopharmaceuticals pays non établi dans la noticeUniversité ou école supérieure
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Jiangsu Provincial Novel Anti-Tumor Targeted Drug Conjugate Engineering Research Center pays non établi dans la noticeStructure de recherche
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College of Life Science and Technology Key Laboratory of Molecular Biophysics of the Ministry of Education pays non établi dans la noticeUniversité ou école supérieure
Suzhou Chien-Shiung Biopharmaceutical Industry Think Tank — Suzhou Chien-Shiung Institute of Technology, Huazhong University of Science and Technology et College of Biopharmaceuticals, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.