In vitro organogenesis of banana Cv. Konkan safed velchi by using male floral organ

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An efficient approach for in vitro organogenesis of banana Cv. Konkan safed velchi was standardized utilizing male floral organs viz meristems and fingers. Sterilization treatment with 70% ethanol (60 sec), 10% sodium hypochlorite (10 min) and 0.2% mercuric chloride (6 min) yielded the highest aseptic cultures with 88.67% in meristems and 83.33% in fingers explants. MS medium supplemented with 2.0 mg/l 2,4-D and 0.5 mg/l NAA induces maximum callus (58.67%) in meristems derived explants within 46.67 days. Shoot regeneration was also observed highest (83.67%) on MS medium supplemented with 4.5 mg/l BAP in meristems. Fingers derived explants resulted low response for induce callus (24%) and shoot regeneration (73.00%) on same medium. MS medium supplemented with 5.0 mg/l BAP and 1.0 mg/l kinetin shows highest multiplication rate (8.33). Successfully rooting (87.67%) was observed on medium supplemented with 2.0 mg/l IBA and 0.5 mg/l NAA. This standardized protocol offers a route to large-scale, disease-free multiplication of banana Cv. Konkan safed velchi.

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