Plasmonic investigation of Δ9-Tetrahydrocannabinol Effects onto Neural Activity

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Cannabinoids, particularly the psychoactive compound Δ9-tetrahydrocannabinol (THC), have gained attention for their medical applications, with effects that are based on both age and dosage. In the nervous system, which originates from the ectoderm, both CB1 and CB2 receptors are present in neural stem and progenitor cells, where they are involved in controlling self-renewal, proliferation, and differentiation processes. However, THC effects are not fully characterized in different ages and neuron cells we investigate the effect of THC on the activity of the neural progenitor cells (NPCs) at three different ages (newborn, 1 year, and 2 years). We induce a two-dimensional (2D) plasmonic chip in a ring shape using soft polydimethylsiloxane (PDMS) as a flexible and biocompatible substrate. The micro rings of the 2D plasmonic chip were patterned using Laser writing and then coated with a thin layer of gold (35 nm). To evaluate the chip’s characteristics, a near-field plasmonic setup was employed. The results show a favorable physical phenomenon known as plasmon-induced transparency (PIT). Subsequently, the cultured neural progenitor cells (NPCs) were treated with THC at a concentration of 200 ????g/mL, and their activity was monitored. The results demonstrated the high sensitivity of the plasmonic chip in detecting cellular activity and its response to THC exposure. Furthermore, the measured spectra indicated that neurons derived from aged NPCs exhibited a higher response to THC compared to those derived from newborn cells.

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