Data belonging to PhD thesis chapter 'Towards live cell imaging of dynamic heterogeneous protein collectives'

Résumé fourni par la source

Data underlying Chapter 7 of the PhD thesis by Marieke Glazenburg. In this chapter, a proposed method to visualize heterogeneous protein collectives in vivo was described and characterized in preliminary experiments. Data is mostly live cell imaging data to analyze protein localization and inducible expression induction, as well as plate reader data for extracting dose response curves. The dataset contains the following components.Imaging data (one timepoint) of live cells expressing Spa2-TurboID-YFP (folder spa2-turboID-YFP_live, Figure 7.2) The purpose of this experiment was to confirm correct localization of Spa2-TurboID.Imaging data of AlexaFluor405 signal in fixed cells expressing Spa2-TurboID-YFP stained with streptavidin-AlexaFluor405 (folder streptavidin_staining, Figure 7.3) The purpose of this experiment was to identify regions enriched for biotinylation.Plate reader output of the GFP induction dose response assay (folder dose_response_essay, Figure 7.5, S7.1 and S7.2) The purpose of this experiment was to find the tunable region of expression induction.Imaging data (timelapses) of cells inducibly expressing yeGFP or mSA-sfGFP (folders yeGFP_induction and mSA-GFP_induction respectively, Figure 7.6) The purpose of this experiment was to characterize expression induction in live cells.Imaging data of YFP signal in fixed cells expressing Spa2-TurboID-YFP (folder spa2-turboID-YFP_fixed, Figure 7.7) The purpose of this experiment was to verify maintenance of native fluorescence in fixed cells.Code used to analyze the dose response assay (Fig. 7.5, S7.1 and S7.2) and live cell yeGFP expression (Figure 7.6) (folder code).Plasmid maps of all plasmids used in the study All methods and experimental details are described in detail in the chapter.

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