Figure 4 from Glioblastoma Subtypes Exhibit Distinct Migration Mechanics and Immune Responses

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Cancer cell migration is subtype specific and independent of the tumor microenvironment and species. A, Six representative images of primary isolated cells from each of the two mouse tumor subtypes (3 NRAS and 3 PDGF). GFP-tagged tumor cells (green) and blood vessels staining using isolectin B4 (magenta). B, NRAS/mesenchymal primary isolated tumor cancer cells migrate faster than PDGF/proneural primary isolated cancer cells in normal mouse brain tissue as measured by random motility coefficient, see Supplementary Video S2. C and D, Quantification of cell spread area and cell aspect ratio showing that NRAS/mesenchymal cancer cells are more spread (C) and tend to be somewhat more polarized (D), P = 0.075, than PDGF/proneural cancer cells in healthy brain slices. E, Six representative images of proneural and mesenchymal PDX lines cells in healthy mouse brain tissue. Labeled tumor cells using DiO membrane dye (green) and blood vessel staining using isolectin B4 (magenta). F, Mesenchymal PDX cells migrate faster than proneural PDX cells in normal mouse brain tissue as measured by random motility coefficient, see Supplementary Video S3. G and H, Quantification of cell spread area and cell aspect ratio showing that mesenchymal PDX cells are more spread (G) and more polarized (H) than proneural PDX cells in healthy brain slices. Solid and dashed lines represent mean and median values, respectively. Error bars represent the SEM. +, P < 0.05; *, P < 0.01; **, P < 0.001; ***, P < 0.0001; ****, P < 0.00001. Statistical significance was assessed using the Kruskal–Wallis rank test.

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