Homoeolog expression divergence contributes to time of day changes in transcriptomic and glucosinolate responses to prolonged water limitation in B. napus

Le résumé fourni par la source

Included are the raw ('.raw') files that have not been manipulated in any way. We provide these files instead of (ex) .mzML files so interested parties may follow the workflow I provide in the methods, and/or create their own preferred workflows with these data. This does mean that the user will have to do a bit of converting to process the raw data. Assuming the user wishes to identify features of interest using mzMine they will first have to convert the raw data to a .mzML format using msConvert (https://mzmine.github.io/mzmine_documentation/data_conversion.html). These data were collected in negative mode and centroided using the CWT algorithm in msConvert. Due to the large size of this dataset (4-5 biological replicates * 3-5 technical replicates * 3 time points * 2 treatments * 2 accessions * 3 positional tissue punches) the files were compressed in chunks so all 7 compressed files must be downloaded for the entire dataset. For the paper we analyzed the 'tip' samples (a hole punch collected at the tip of each leaf); we also provide the raw data for a punch collected at the base of the leaf, to the right of the midvein and tangental to the leaf edge ("tan1" samples), as well as another punch directly above this one ("tan2). We also include an RMarkdown file and the associated data used to run all of the analyses and make the figures. For further details see the methods section of the attached paper.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.