Figure 4 from Tumor-Associated Platelets Suppress T-cell Function and Promote Immune Evasion in TNBC via the P-selectin/P-selectin Glycoprotein Ligand 1 Pathway

Le résumé fourni par la source

T-cell function preserved with blocked TAP–T-cell interaction through introduction of crizanlizumab. Flow cytometric quantification of platelet–T-cell binding in both (A) CD4+ and (B) CD8+ effector T-cell populations. Quantification of the functionality of T cells cocultured with tumor-associated platelets (TAP) and crizanlizumab or RB40.34 in both (C) CD4+ and CD8+ (D) effector T cells and exhaustion of (E) CD4+ and (F) CD8+ effector T cells. Levels of Zombie NIR caspase 3/7 dye detected over 48 hours. Flow cytometry was used to detect death in (G) CD3+ T cells and (H) AT-3 cells after T cells were previously cocultured with TAPs and crizanlizumab. Data are representative of two independent experiments. Statistical significance was determined using a one-way ANOVA followed by the Tukey multiple-comparison test. ns, not statistically significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

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