Author response: Optimised genome editing for precise DNA insertion and substitution using prime editors in zebrafish

Le résumé fourni par la source

eLife AssessmentThis study presents a comparison of the efficiency and precision of two prime editing methods to introduce single-nucleotide variants and longer exogenous DNA sequences into the zebrafish genome.Convincing data support the conclusion that the PE2 prime editor Nickase is more effective at introducing single-nucleotide variants, while the PEn prime editor nuclease is more effective at integrating sequences from 3 up to 46 base pairs, for both somatic and germline editing.The results will be valuable for the zebrafish community, in particular to model human disease variants in this model organism.

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