SIFT-MS volatilome fingerprinting for rapid screening of aroma precursor potential in grapes: a case study on Vitis vinifera and hybrid varieties

Le résumé fourni par la source

The increasing development of disease-resistant hybrid grape varieties requires rapid analytical approaches to assess their aroma potential. Conventional methods used to quantify glycosidic aroma precursors rely on laborious extraction procedures and GC–MS analyses. In this study, Selected Ion Flow Tube Mass Spectrometry (SIFT-MS), a high-throughput headspace analysis technique, was evaluated as a rapid volatilome fingerprinting tool to screen the aroma precursor potential of grape varieties. Thirty-three samples of red Vitis vinifera and hybrid frozen berries were thawed, homogenized in tartrate buffer (pH 5.5), incubated at 30°C for 15 h either without or with β-glycosidase, and analyzed by SIFT-MS using O 2 ⁺ as reagent ion. The volatilome showed clear clustering driven by enzymatic hydrolysis, highlighting the influence of glycosidic precursors on volatile signatures. A distinct cluster was observed for Clinton samples ( V. riparia × V. labrusca ). A classification and regression tree (CART) model developed from enzyme-treated samples showed promising ability to predict total glycosidic precursor content measured by GC–MS (R² = 0.94; RMSECV = 218 µg/L). Despite the limited influence of variety type, several low m/z ions enabled accurate sample classification and revealed diagnostic markers. These results highlight the potential of SIFT-MS as a rapid tool for screening aroma precursor potential in grapes. The proposed workflow reduces analytical turnaround to 18–20 hours per batch, compared with 2–3 days for conventional methods. As a trade-off between throughput and analytical certainty, it is compatible with the pace of harvest operations and large-scale variety phenotyping. It is potentially transposable to other plant matrices in which glycosidic precursors play a role in flavor development.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.