Figure 3 from A Conserved Enhancer Locus in Extrachromosomal DNA and Homogeneously Staining Regions Activates MYC Transcription in Group 3 Medulloblastoma

Résumé fourni par la source

ecMYC E1 displays canonical enhancer hallmarks in MYC-amplified G3-MB. A–C, H3K27ac-anchored Hi-ChIP contact map shows H3K27ac-mediated looping interactions between the MYC promoter (yellow marker) and the ecMYC E1 locus (blue marker) (identified by the black box) along with H3K27ac occupancy by ChIP-seq (represented by the blue sequencing track) at the ecMYC E1 locus in (A) D425, (B) D458, and (C) HDMB03. D, Aligned ATAC-seq track heatmap of MYC-amplified G3-MB: D283, D341, D425, D458, HDMB03, SJMB016880, and SJMB049281; non-MB MYC-amplified: COLO320HSR and COLO320DM; MYC-overexpressing DIPG: DIPG007, DIPG13, DIPG19, DIPG24, and DIPG25; and MYC-overexpressing solid tumors: RH30, RD, and SMSCTR. E, Copy-number variation for the MYC promoter (y-axis) versus copy number of the ecMYC E1 enhancer (x-axis) across MYC-amplified cancers: G3-MB (pink dots), all other tumor types (black dots). F, ATAC-seq tracks from cells of the developing human cerebellum at the 8q24 locus (48).

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.