Transcriptome analysis of goat adipose tissue-derived mesenchymal stem cells cultured in variable oxygen conditions

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Introduction: Oxygen tension influences mesenchymal stem cell biology, but the transcriptional responses of goat adipose tissue-derived mesenchymal stem cells (gADSCs) to different oxygen-exposure conditions remain incompletely understood. Methods: RNA sequencing (RNA-seq) was used to investigate oxygen-dependent transcriptional responses in gADSCs cultured under normoxia (NO), sustained hypoxia (HO), and transient hypoxia (THO). Differentially expressed genes (DEGs), enriched biological processes, and protein-protein interaction networks were analysed. Selected DEGs and hub/bottleneck genes were validated by quantitative reverse transcription PCR (RT-qPCR). Results: The analysis identified condition-associated gene expression changes and candidate pathways related to cell-cycle regulation, DNA repair, extracellular matrix organisation, inflammatory response, pH regulation, angiogenic signalling, and hypoxia-inducible factor-associated adaptation. RT-qPCR validation further revealed differential regulation of hypoxia-inducible factor 1-alpha (HIF1A) and hypoxia-inducible factor 2-alpha (HIF2A), suggesting possible divergence between acute and sustained hypoxic responses. Discussion: These findings provide a transcriptomic resource for understanding oxygen-dependent regulation of gADSCs. However, protein-level validation and functional assays are required to confirm the biological roles of the prioritised genes and assess their relevance to regenerative applications.

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