Metabolite Profiling of Volatile and Non-Volatile Compounds in Citrus Varieties under Huanglongbing Stress Following Bio-Enzyme Treatment

Résumé fourni par la source

Huanglongbing (HLB) disease is caused by Candidatus Liberibacter asiaticus (CLas), which is a bacterium that affects citrus metabolism, impacting fruit quality and yield. This study determined the effectiveness of a bio- enzyme that was prepared in the lab to help correct the metabolic disturbances that HLB causes in six varieties of Citrus limon and Citrus sinensis. The volatile and non-volatile metabolites were analysed by GC-MS, and significant changes in metabolites, such as limonene, linalool, alpha-terpineol or other stress factors like methyl salicylate, aldehyde and others, were found. Changes in the levels of metabolites by HLB infection were quantified by qPCR analysis. Bio-enzyme treatment resulted in a reduction in CLas titers and partially corrected metabolic stability by increase of defence associated metabolites, such as γ-terpinene, β-caryophyllene, ferulic acid, naringenin and chlorogenic acid. The principal component analysis showed a clear separation between treated and untreated plants, while ANOVA showed significant differences between cultivars and treatments (p < 0.05). Based on these results, it can be concluded that bioenzymes can be used as a mutation for better citrus plant tolerance toward HLB-infection that can be considered as a sustainable management practice.

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