Automatic QuEChERS combined with liquid chromatography-mass spectrometry for determination of 172 pesticides and their metabolite residues in feed

Le résumé fourni par la source

The experiment aimed to explore the determination of residues of 172 pesticides and their metabolites in feed using automatic QuEChERS combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Feed samples were crushed and soaked in water, then extracted with acetonitrile and purified with N-propyl ethylene diamine (PSA) and C18. The one-stop pretreatment was carried out by the automatic QuEChERS sample preparation system. Quantification was performed using the matrix-matched external standard method under the positive and negative ion modes of electrospray ionization. The results showed that the correlation coefficients (r) of the 172 pesticides and their metabolites were all >0.99 in the range of 0.002~0.100 mg/L, and the limit of quantification reached 0.01 mg/kg. The average recoveries (relative standard deviations) at the spiked levels of 0.01, 0.02 and 0.10 mg/kg were 63.3%~104.0% (1.02%~12.30%), 66.5%~104.0% (0.34%~10.20%) and 80.1%~108.0% (0.37%~12.30%), respectively. The study indicates that the LC-MS/MS method for detecting the residues of 172 pesticides and their metabolites in feed, which uses automatic QuEChERS technology for extraction and purification and the external standard matrix-matched standard curve method for quantification, has the characteristics of simple operation, rapidity, and high degree of automation, and is suitable for the rapid analysis of multiple pesticide residues in feed.

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