Additional file 3 of Centrosomal protein 72 deficiency exacerbates liver fibrosis induced by Schistosoma japonicum infection

Résumé fourni par la source

Additional file 3. Validation of EGR1 overexpression and CEP72 knockdown in 293T cells. (A) qRT-PCR analysis of EGR1 mRNA expression in 293T cells transfected with empty vector (pcDNA3.1) or EGR1 expression plasmid (pcDNA3.1-EGR1). (B) Western blot analysis of EGR1 protein levels in 293T cells transfected with pcDNA3.1 or pcDNA3.1-EGR1; GAPDH was used as a loading control. (C) qRT-PCR analysis of CEP72 mRNA expression in 293T cells transfected with control shRNA (shNC) or CEP72-targeting shRNAs (shCEP72#1 and shCEP72#2). Data are presented as mean ± SD from three independent experiments. Statistical significance was assessed using an unpaired two-tailed Student’s t-test for two-group comparisons (A) and one-way ANOVA for multiple-group comparisons (C). P < 0.05 was considered statistically significant (**** P < 0.0001).

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.