Discrimination of Trout Fed with Traditional and Insect-Based Diets by GC–MS and MOX Sensors: Influence of Cooking on Volatile Profiles

Le résumé fourni par la source

The use of insect-based protein sources in aquaculture is gaining increasing attention with Hermetia illucens (black soldier fly, BSF) larvae meal representing a promising substitute to fishmeal (FM). This study evaluated the effect of partial dietary inclusion of BSF meal (BSF0, BSF2.5, BSF5, BSF10%) on the volatilome of rainbow trout (Oncorhynchus mykiss) fillets, before and after cooking, using gas chromatography–mass spectrometry (GC–MS) and a metal oxide sensor-(MOX)-based device. Fish were fed diets with increasing BSF inclusion, and both raw and cooked fillets were analyzed to assess changes in volatile organic compounds (VOCs). GC–MS enabled the identification and semi-quantitative analysis of VOC classes, while MOX sensor responses were processed using Linear Discriminant Analysis (LDA) to assess discrimination among dietary treatments. Results showed that BSF inclusion influenced the volatile profile, with clearer separation at higher inclusion levels (BSF5–BSF10%), especially in cooked fillets. Thermal processing enhanced these differences. GC–MS analysis revealed a reduction in aldehydes and ketones and an increase in carboxylic acids with higher BSF inclusion. Key compounds such as hexanal and heptanal decreased, indicating changes in lipid-derived volatile pathways. Overall, the integration of GC–MS and MOX sensors proved effective in detecting diet-induced changes, supporting their application as effective and reliable tools for quality assessment in aquaculture products, with potential implications for sensory quality that should be further confirmed through dedicated sensory studies.

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