Supplementary Figure S2 from Neutrophils Mediate Protection Against Colitis and Carcinogenesis by Controlling Bacterial Invasion and IL22 Production by γδ T Cells

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Figure S2. Neutrophil deficiency is associated with intestinal dysbiosis. Related to Figure 2 A) Representative histological images of H&E-stained colon section from Csf3r+/+(left) and Csf3r-/- (right) mice, after one cycle of DSS, showing the increased inflammatory infiltrate and the presence of bacterial accretions (indicated by black arrow) in Csf3r-/-. B-C) Shannon Index representing the evenness in the overall number of bacterial species between wild-type and neutrophil deficient mice untreated (B) and DSS-treated mice (C). D-E) Taxonomic analysis of the phyla that compose the microbiota of feces of untreated (D) and DSS-treated (E) Csf3r+/+ (untreated n=4, DSS-treated n=3) and Csf3r-/- (untreated n=4, DSS-treated n=4) mice. F) Microbiota depletion efficiency measured by qPCR for 16S gene: complete depletion is achieved after 20 days of antibiotic treatment. G) Body weight loss during DSS-induced acute colitis in Csf3r+/+(n=11) and Csf3r-/- (n=9) mice with and without cohousing (Csf3r+/+Cohoused n=5 and Csf3r-/-Cohoused n=5). H) Body weight loss of AOM/DSS treated Csf3r+/+(n=7) and Csf3r-/- (n=5) mice with and without cohousing (Csf3r+/+Cohoused n=5 and Csf3r-/-Cohoused n=5); I) Macroscopic polyp count at the experimental endpoint. A-I) One experiment. B-C) Unpaired Student’s t-Test. F, I) Multiple Student’s t-Test. G-H) Wilcoxon matched-pairs signed rank test. Data are mean ± SEM. *** p < 0.001 ** p < 0.01 * p < 0.05.

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