Effect of Low Nitrogen on Photosynthesis, Physiology, and Mineral Element Responses of Self-Grafted and Grafted Citrus Seedlings

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Grafting is a widely used technique to improve stress tolerance in horticultural plants. However, little is known about how grafting affects citrus growth under low-nitrogen (N) stress. To investigate the responses of different grafting combinations to low N availability, we examined root morphology, photosynthesis, chlorophyll fluorescence and semi-quantitative mineral profiles in grafted and ungrafted citrus plants subjected to two N levels (10 and 0.15 mM NO3− -N) under potted conditions. Analyses were performed on roots and leaves of six plant combinations: ungrafted Trifoliate orange (Poncitrus trifoliata L. Raf., Pt) and red tangerine (Citrus reticulata Blanco, Cr); self-grafted combinations (Pt/Pt and Cr/Cr); and reciprocal heterografts (Pt/Cr and Cr/Pt). Under low-N stress, plant height decreased by 12.3–36.8%, stem diameter by 2.9–31.8%, leaf area by 18.2–26.3%, and SPAD by 11.6–24.5% across the six combinations, with the Cr/Cr combination showing the largest reductions in all parameters. The highest net photosynthetic rate (Pn), intercellular CO2 concentration (Ci), stomatal conductance (Gs), electron transport rate (ETR), maximum quantum efficiency of PSII (Fv/Fm) and effective quantum efficiency of PSII (Fv’/Fm’) were observed in the Pt and Pt/Pt plants. Low-N stress reduced chloroplastid pigment contents and limited photosynthetic rates. Under 10 mM N treatment, the Fv/Fm values of Pt, Cr, Pt/Pt, and Cr/Pt were approximately 0.82, whereas those of Pt/Cr and Cr/Cr were below 0.82, suggesting lower maximal PSII efficiency in combinations with Cr rootstock. Regarding mineral elements, under low-N stress, the relative levels of P, K, Ca, Mg, and Fe in leaf and root sap increased, while those of N, Cu, Zn, B, and Mn decreased. Overall, combinations with Pt rootstock (Pt/Pt and Cr/Pt) showed better growth and photosynthetic performance, and more stable mineral profiles under low-N stress than combinations with Cr rootstock (Cr/Cr and Pt/Cr). These findings provide a physiological basis for understanding rootstock-specific responses to low-N stress under controlled conditions.

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