MALDI-MSI Single-Cell Lipidomics Datasets of Glioma and Breast Cancer Cell Lines Acquired Using CellenOne-Guided Cell Deposition - Positive ion mode

Résumé fourni par la source

The dataset was generated to evaluate a workflow combining the CellenOne single-cell isolation platform with MALDI-MSI for high-throughput single-cell lipidomic profiling without fluorescence staining, cell registration, or pre-imaging procedures. Cells were individually dispensed onto indium tin oxide (ITO)-coated glass slides using the CellenOne platform and analyzed using a Bruker MALDI timsTOF flex instrument at 10 µm spatial resolution. Data were acquired in both positive ionization mode. The repository includes centroid imzML/ibd20 datasets from the following cell lines: Glioma: NCH82, A172, U118 Breast cancer: MCF7, AU565, BT549 Irradiated NCH82 cells Dataset characteristics Instrument: Bruker MALDI timsTOF flex Spatial resolution: 10 µm Ionization modes: Positive Data format: imzML/ibd These datasets support the development and evaluation of fluorescence-free, registration-free single-cell MALDI-MSI workflows and may be reused for benchmarking, image analysis, lipidomics and computational method development.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.