Figure 2 from CD4+ T Cells Sensitize Quasimesenchymal Breast Tumors Lacking CD73 to Anti-CTLA4 Immune Checkpoint Blockade Therapy

Résumé fourni par la source

CD8+ T cells are partially important for sensitizing qM tumors lacking CD73 to anti-CTLA4 ICB. A, Schema and tumor kinetics for sgCD73 tumor-bearing mice treated with the indicated antibodies. Data represent three independent experiments in which n = 3–4 for each group. B, Representative immunofluorescence images of primary tumor sections obtained from (A) stained for CD4 (red) and DAPI (blue) at 63× magnification. Bar graph on the right represents quantification of CD4+ T cells in each high-power field (HPF) at 63× magnification for the indicated treatment groups. Fields of view (3–5) from the tumor interior were obtained for each tumor. Data represent three independent experiments, with n = 3–5 mice in each group. C, Schema and tumor kinetics for sgCD73 tumors propagated in WT or CD8-KO mice treated with the indicated antibodies. Data represent three independent experiments in which n = 3–4 for each group. D, Schema and tumor kinetics for sgCD73 tumors propagated in WT or B2M-KO mice treated with the indicated antibodies. Data represent three independent experiments in which n = 3–5 for each group. E, Representative immunofluorescence images of primary tumor sections obtained from (C) stained for CD4 (red) and DAPI (blue) at 63× magnification. Bar graph on the right represents quantification of CD4+ T cells in each HPF at 63× magnification for the indicated treatment groups. Fields of view (3–5) from the tumor interior were obtained for each tumor. Data represent three independent experiments, with n = 3–5 mice in each group. F, Representative immunofluorescence images of primary tumor sections obtained from (D) stained for CD4 (red) and DAPI (blue) at 63× magnification. Bar graph on the right represents quantification of CD4+ T cells in each HPF at 63× magnification for the indicated treatment groups. Fields of view (3–5) from the tumor interior were obtained for each tumor. Data represent three independent experiments, with n = 3–5 mice in each group. G, Schema and tumor kinetics for SnailHI qM tumor-bearing mice treated with the indicated antibodies. Data represent two independent experiments in which n = 3–4 for each group. H, Representative immunofluorescence images of primary tumor sections obtained from (G) stained for CD4 (red) and DAPI (blue) at 63× magnification. Bar graph on the right represents quantification of CD4+ T cells in each HPF at 63× magnification for the indicated treatment groups. Fields of view (3–5) from the tumor interior were obtained for each tumor. Data represent three independent experiments, with n = 3–5 mice in each group. A, C, D, and G, Data represent the SEM, two-way ANOVA, **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. B, E, F, and H, Bar graph data represent the SEM, two-tailed unpaired t test, *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Scale bars are 100 μm.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.