In-Vitro Cardioprotective Pharmacological Investigation and Lipid Peroxidation Inhibition Studies of Polyherbal Extract Containing Momordica Charantia Using Oxidative Stress Models

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The present study investigated the in vitro cardioprotective and lipid peroxidation inhibitory activity of a polyherbal extract composed of Momordica charantia, Angelica archangelica, and Hamamelis virginiana using oxidative stress models. The extract was prepared using a hydroalcoholic method and subjected to phytochemical screening, which confirmed the presence of phenolics, flavonoids, tannins, and other bioactive constituents. Quantitative estimation revealed high total phenolic (74.82 mg GAE/g) and flavonoid content (76.39 mg QE/g), indicating strong antioxidant potential. Lipid peroxidation inhibition was evaluated using the TBARS assay in cardiac tissue homogenate, where the extract exhibited significant concentration-dependent activity with 69.05% inhibition at 400 µg/mL. The extract also demonstrated effective scavenging of hydrogen peroxide, superoxide, hydroxyl radicals, and nitric oxide, with notable potency against hydroxyl radicals. The reducing power assay confirmed its electron-donating capacity. The IC₅₀ values across assays indicated moderate to strong antioxidant activity compared to ascorbic acid. The results suggested that the polyherbal extract exerts cardioprotective effects through inhibition of lipid peroxidation, scavenging of reactive species, and stabilization of cellular membranes. These findings support the potential application of the formulation as a natural cardioprotective agent and warrant further in vivo investigation.

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