LncRNA MIR503HG serves as a diagnostic biomarker in patients with sepsis and regulates sepsis‐induced inflammation and cardiac dysfunction
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= 71). A cecal ligation and puncture (CLP) sepsis rat model was constructed. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to evaluate the target gene expression. Enzyme-linked immunosorbent assay was used to measure the levels of myocardial damage markers and inflammatory factors. RNA immunoprecipitation and Dual luciferase assay were used to determine the targeting relationship. Kyoto Encyclopedia of Genes and Genomes analysis was used to predict signaling pathways of target genes. MIR503HG expression was significantly down-regulated in sepsis patients and SICD patients, and it has good diagnostic value for these two types of diseases. Its expression was significantly negatively correlated with myocardial injury markers (cardiac troponin I [cTnI], creatine kinase-MB [CK-MB]), cardiac function indicators (left ventricular ejection fraction), inflammatory factors (interleukin-6, tumor necrosis factor-alpha), and disease severity scores (Sequential Organ Failure Assessment, Acute Physiology and Chronic Health Evaluation II). Furthermore, it exerted a protective effect in sepsis: MIR503HG overexpression could effectively alleviate SICD and mitigate the inflammatory response, as evidenced by decreased left ventricular end-diastolic pressure, increased left ventricular systolic pressure, reduced levels of CK-MB and cTnI, as well as restored myocardial systolic/diastolic capacity (maximal rate of left ventricular pressure rise/fall [±dP/dt]). Mechanistically, MIR503HG functions as a molecular sponge that sequesters microRNA-497-5p (miR-497-5p), thereby lifting brain-derived neurotrophic factor (BDNF). Delivery of miR-497-5p-agomiR partially offset the cardioprotective and anti-inflammatory effects of MIR503HG, whereas overexpression of BDNF partially restored them. MIR503HG relieved sepsis and reduced cardiac dysfunction and inflammatory response by regulating the miR-497-5p/BDNF axis.
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DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- LncRNA MIR503HG serves as a diagnostic biomarker in patients with sepsis and regulates sepsis‐induced inflammation and cardiac dysfunction
- Date Crossref
- 26/05/2026
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
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The Second Nanning People's Hospital pays non établi dans la noticeÉtablissement de santé
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Ruikang Affiliated Hospital of Guangxi Medical University pays non établi dans la noticeÉtablissement de santé
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Harbin Medical University pays non établi dans la noticeUniversité ou école supérieure
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Second Affiliated Hospital of Harbin Medical University pays non établi dans la noticeÉtablissement de santé
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Blood Transfusion Department The Second Affiliated Hospital of Guangxi Medical University Nanning China pays non établi dans la noticeUniversité ou école supérieure
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Department of Intensive Care Unit Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine Nanning China pays non établi dans la noticeUniversité ou école supérieure
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Emergency Department Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine Nanning China pays non établi dans la noticeUniversité ou école supérieure
The Second Nanning People's Hospital, Ruikang Affiliated Hospital of Guangxi Medical University et Harbin Medical University, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.