Prion-like domains control plastid targeting of PEPSI effectors and PEPSI6-mediated modulation of DXR during root symbiosis
Rattachement africain : de. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Abstract Microbial effectors often act in the apoplast or cytosol, but how they reach host organelles during beneficial plant–fungal interactions remains poorly understood. Here, we identify a class of secreted prion-like effector proteins from the mutualistic root endophyte Serendipita indica , termed PEPSIs. These proteins contain prion-like domains (PrLDs) embedded within intrinsically disordered regions, and three tested PEPSIs require these domains for plastid localization. Focusing on PEPSI6, we show that plastid targeting depends on its PrLD and is associated with engagement of plastid import and proteostasis machinery. In plastids, PEPSI6 associates with 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR), promotes DXR accumulation, alters methylerythritol phosphate pathway metabolites, and enhances tolerance to the DXR inhibitor fosmidomycin. PEPSI6 also undergoes apoplastic C-terminal CAP-domain processing, and its colonization-promoting activity persists after PrLD deletion, indicating a plastid-targeting-independent function. Overall, this work identifies PrLDs as noncanonical plastid-targeting elements in PEPSI effectors and reveals DXR as a target of fungal manipulation during root symbiosis. Highlights Prion-like domains control plastid targeting of selected PEPSI effectors. PEPSI6 associates with DXR and promotes its accumulation in plastids. PEPSI6 alters MEP-pathway metabolites and increases tolerance to fosmidomycin. Apoplastic processing reveals a plastid-independent PEPSI6 activity during root symbiosis.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Prion-like domains control plastid targeting of PEPSI effectors and PEPSI6-mediated modulation of DXR during root symbiosis
- Date Crossref
- 18/05/2026
- Éditeur
- openRxiv
- Type
- posted-content
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
University of Cologne Institute for Plant Sciences pays non établi dans la noticeUniversité ou école supérieure
-
Cluster of Excellence on Plant Sciences pays non établi dans la noticeStructure de recherche
-
Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases pays non établi dans la noticeStructure de recherche
-
University Hospital Cologne pays non établi dans la noticeÉtablissement de santé
-
Max Planck Institute for Plant Breeding Research pays non établi dans la noticeStructure de recherche
-
Leibniz Institute of Plant Biochemistry Program Center MetaCom pays non établi dans la noticeStructure de recherche
-
Faculty of Medicine Institute for Integrated Stress Response Signaling pays non établi dans la noticeUniversité ou école supérieure
Institute for Plant Sciences — University of Cologne, Cluster of Excellence on Plant Sciences et Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.