Additional file 1 of Efficacy and safety evaluation of artificial intelligence-identified antimicrobial peptides targeting avian pathogenic Escherichia coli in broiler chickens

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Additional file 1: Table S1. Antimicrobial peptide (AMP) and dosage tested on two chicken breeds. Table S2. Additional in vitro testing for select AMPs. Table S3. qPCR assay primer/probe sequences. Table S4. qPCR reaction conditions. Table S5. qPCR assay efficiencies by assay and tissue type. Table S6: Hatchability of eggs in APEC challenge trials. Fig. S1. Kaplan–Meier survival plots of chickens treated with 20 μg TeRu4, TeBi1, or PeNi4. Fig. S2. Kaplan–Meier survival plots of birds treated with either 1, 5, or 10 µg AMP. Fig. S3. Weight gain of chickens treated with TeRu4 and PeNi4. Fig. S4. APEC infection percentages in birds across multiple post-hatch time points and tissues. Fig. S5. Relative fold change of cytokine transcripts in the cecal tonsil of APEC challenged birds. Fig. S6. Relative fold change values of cytokine transcripts in the spleen of APEC challenged birds. Fig. S7. Relative fold change of IL-1β, IL-8, and IL-6 transcripts lipopolysaccharide (LPS)-treated HD11 cells. Fig. S8. Relative fold change of IL-1β, IL-8, and IL-6 transcripts AMP-preincubated HD11 cells. Fig. S9. Raw percentage values for hatchability parameters across various AMPs and dosages. Fig. S10. Kaplan–Meier curves survival plots of the bird sexes on d 10 of the pen trials. Fig. S11. Kaplan–Meier survival plots of control and treatment groups on d 10 of the pen trials. Fig. S12. Kaplan–Meier survival plots of the bird sexes on d 35 of the pen trials. Fig. S13. Kaplan–Meier survival plots of experimental groups on d 35 of the pen trials. Fig. S14. Mean weights of female and male birds during pen trials across different AMPs and dosages. Fig. S15. Feed conversion ratio (FCR) of male and female birds during pen trials. Fig. S16. Flock uniformity of female and male birds during pen trials across different AMPs and dosages. Fig. S17. Relative cytokine transcripts in the spleens of pen trial birds treated with 10 µg TeBi1. Fig. S18. Stability of TeRu4 and Tebi1 in sterile water over 14 d.

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