Inefficacy of dexamethasone and pirfenidone as comparators in the bleomycin-induced pulmonary fibrosis model in SD rats
Rattachement africain : ru, us, mn. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Background: Pulmonary fibrosis (PF) is a severe disease leading to disability and death. It is important to search for novel therapeutic agents, for which a model of bleomycin-induced PF is used and pirfenidone and dexamethasone are used as comparators. The aim of the study was to establish the effect of comparators on model PF. Methods: We induced PF in male SD rats by intratracheal administration of 2.5 mg/kg bleomycin (BLM). Dexamethasone was administered intravenously (0.5 mg/kg, 7 days,), and pirfenidone was administered orally (50 mg/kg, 21 day). Clinical signs of health deviations, body weight, weight gain, food consumption and spirometry were monitored. The lung condition, cellular composition of the bronchoalveolar fluid (BALF), the lung concentration of hydroxyproline, blood biochemistry and the severity of fibrosis were analyzed 8 and 22 days post PF induction. Results: All animals administered BLM intratracheally developed pronounced signs of PF, including weight loss leading to pulmonary cachexia, deterioration in general condition, increased respiratory rate and decreased vital capacity, increased hydroxyproline levels in lung tissue, elevated level of neutrophils, lymphocytes, and pulmonary macrophages in the BALF. All of these features developed over time and were evident on day 8 and 22 of the study. Histological analysis revealed a pronounced inflammatory response on day 8 and a generalized fibrotic process on day 22. Dexamethasone and pirfenidone not only failed to improve clinical condition of animals, but even worsened it; one animal receiving pirfenidone died. Conclusion: Animals with PF treated with dexamethasone and pirfenidone showed no improvement compared to untreated animals. Although dexamethasone reduced hydroxyproline levels, this did not improve either the animals' general condition or lung damage.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Inefficacy of dexamethasone and pirfenidone as comparators in the bleomycin-induced pulmonary fibrosis model in SD rats
- Date Crossref
- 11/05/2026
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Institute of Bioorganic Chemistry pays non établi dans la noticeStructure de recherche
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Patara Pharma (United States) pays non établi dans la noticeEntreprise
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Peoples' Friendship University of Russia pays non établi dans la noticeUniversité ou école supérieure
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Ministry of Health of the Russian Federation pays non établi dans la noticeOrganisme public
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Ministry of Health pays non établi dans la noticeOrganisme public
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Moscow Center for Advanced Studies pays non établi dans la noticeInstitution
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Valenta Pharm JSC pays non établi dans la noticeInstitution
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Russian University of Medicine Department of Biological Chemistry pays non établi dans la noticeUniversité ou école supérieure
Institute of Bioorganic Chemistry, Patara Pharma (United States) et Peoples' Friendship University of Russia, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.