Impact of Polishing and Finishing Techniques on Resin-based Restorative Materials Cytotoxicity Using Real-time Cell Analysis

Le résumé fourni par la source

INTRODUCTION: Resin-based restorative materials (RBRMs) are extensively used in contemporary dentistry; however, their biocompatibility remains a concern due to the release of residual monomers. Finishing-polishing procedures may significantly influence surface characteristics and subsequent cytotoxic behavior, yet dynamic evidence using the real-time cell analysis (RTCA) is limited. This research was done to evaluate the outcome of different finishing-polishing procedures on the cytotoxicity of RBRMs using RTCA. MATERIALS AND METHODS: This in vitro experimental study included 200 disc-shaped specimens fabricated from nanohybrid, bulk-fill, and microhybrid composite resins. Specimens were categorized into four groups depending upon surface treatment: Mylar strip (control), diamond bur finishing, disc polishing and multi-step polishing. Human gingival fibroblasts were exposed to material extracts and cellular responses were used with the xCELLigence RTCA system to monitor them constantly for 72 h. One-way ANOVA and Tukey's post hoc test (P < 0.05) were used for the statistical analysis. RESULTS: RTCA demonstrated a significant time-dependent and surface-treatment dependent cellular response. Multi-step polishing exhibited the highest cell viability, followed by disc polishing and diamond bur finishing. While the control group showed the lowest cell index values (P < 0.05). Nanohybrid composites demonstrated significantly higher biocompatibility compared to bulk-fill and microhybrid materials. CONCLUSION: Finishing-polishing procedures and material composition significantly influence the cytotoxic behavior of RBRMs. Optimized polishing protocols enhance cellular viability and improve biological safety.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.