Postnatal Reprogramming of Human Intestinal Epithelium

Le résumé fourni par la source

This repository contains processed single-cell and spatial transcriptomics datasets generated as part of a comprehensive study of human intestinal development as well as validation IHC and ISH images. The data include Seurat RDS objects for: (i) MERSCOPE spatial transcriptomics data, and (ii) epithelial Seurat objects from colon and ileum single-cell multi-omics datasets. The underlying study profiles human intestinal tissues across fetal, neonatal and pediatric stages, integrating single-cell RNA expression and chromatin accessibility with high-resolution spatial transcriptomics. Across >200,000 single cells and >1 million spatially resolved cells, the dataset captures epithelial, immune and stromal compartments and defines age-specific cellular states, regulatory programs and spatial organization of the developing gut . These RDS objects contain cell embeddings, cell type annotations and sample meta data stored in the @meta.data slot. Multiome data also contains "peaks" assay, which stores ATAC part of the dataset. IHC images directory contains BHLHE40 immunohistochemistry images across age groups. ISH images directory contains BHLHE40 RNA in situ hybridisation images across age groups.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.