Aeroponics in the characterization of the root system of eucalyptus

Le résumé fourni par la source

Understanding the root system of plants is essential for improving productivity and resilience, particularly under water-deficit conditions. Despite its recognized importance, root system characterization in eucalyptus has been limited. This study presents a fast and accurate method for evaluating root biomass in eucalyptus seedlings and cuttings using an aeroponic system. Custom-built aeroponic boxes equipped with external drip irrigation and internal fogger systems were developed to accommodate 90-day-old seedlings/cuttings. Initially, the optimal number of replications for aeroponic experiments was determined using 12 vegetatively propagated Eucalyptus spp. clones. This optimization enhanced experimental precision and reproducibility. Subsequently, root system of 23 clonal genotypes and seedlings from seven Eucalyptus spp. species were characterized. After 50 days in the aeroponic system, the following root parameters were evaluated: dry weight, length, and number of roots. Among these, dry root biomass showed the greatest variation among clones, making it a key trait for genotype differentiation. For seed-derived seedlings, root length, as a non-destructive characteristic, makes it possible to select and maintain the superior genotypes in the Breeding Program. This study demonstrates that early root phenotyping using aeroponics is a valuable approach for identifying productive and drought-tolerant eucalyptus clones and species, contributing to more efficient and resilient forestry practices.

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