Chemical composition of Callistemon subulatus essential oils and the protective effects against cyclophosphamide-induced lung toxicity: GC/MS metabolic profiling with in vivo and in silico experimental studies
Rattachement africain : Égypte, sa. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Callistemon is a genus of aromatic plants indigenous to Australia and widely spread for ornamental purposes. In this study, we aimed to investigate the chemical composition of essential oils isolated from the leaves, flowers, stems, and fruits of Callistemon subulatus using gas chromatography–mass spectrometry (GC/MS) analysis, alongside the assessment of the protective effects of the leaf oil against cyclophosphamide (CY)-induced lung toxicity in rats. The leaf oil was rich in oxygenated monoterpenes (51.15%), while the flower oil contained a higher proportion of monoterpene hydrocarbons (72.06%). The stem and fruit oils were predominantly composed of hydrocarbons, accounting for 52.79% and 62.52%, respectively. Interestingly, C. subulatus leaf oil (CLO) showed the highest yield with the highest proportion of eucalyptol (44.36%), a reported anti-inflammatory monoterpene. Thirty rats were divided into five groups and treated for 10 days as follows: group I is a vehicle group, group II was administered CY (200 mg/kg, i. p. ), groups III and IV received oral CLO (50 and 100 mg/kg, respectively) in addition to CY (200 mg/kg, i. p. ), and group V received only CLO (100 mg/kg, oral). It was found that CLO inhibited the lipid peroxidation level and augmented catalase and glutathione levels. Furthermore, it significantly reduced the overtranscription of α -smooth muscle actin ( α- SMA) by preventing fibroblastic cell differentiation into myofibroblasts. The histological appearance of CY-induced lung tissue revealed congestion, epithelial hyperplasia, and inflammatory cell aggregations. However, CLO considerably alleviated these manifestations. The findings demonstrated the preventive function of CLO against the harmful effects of CY on rat lungs. Therefore, C. subulatus oil might be a promising natural candidate for protecting the lung from CY-induced pneumotoxic manifestations.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Chemical composition of Callistemon subulatus essential oils and the protective effects against cyclophosphamide-induced lung toxicity: GC/MS metabolic profiling with in vivo and in silico experimental studies
- Date Crossref
- 14/04/2026
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Ain Shams University Égypte (code pays fourni par la source)Université ou école supérieure
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New Giza University New Giza University, Égypte (code pays fourni par la source)Université ou école supérieure
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King Khalid University pays non établi dans la noticeUniversité ou école supérieure
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Kafrelsheikh University Kafrelsheikh University, Égypte (code pays fourni par la source)Université ou école supérieure
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Faculty of Pharmacy Department of Pharmacognosy Kafrelsheikh University, Égypte (ville ou établissement reconnu, pays non nommé)Université ou école supérieure
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School of Pharmacy Biology Department pays non établi dans la noticeUniversité ou école supérieure
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College of Medicine Department of Medical Physiology pays non établi dans la noticeUniversité ou école supérieure
Ain Shams University (Égypte), New Giza University (New Giza University, Égypte) et King Khalid University, avec 4 autres affiliations. Pays d’affiliation : Égypte.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.