Regenerative effects of myogenic gene transfected MSC derived exosomes on radiation esophagitis
Rattachement africain : kr, pr. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
BACKGROUND: Radiation esophagitis is a common adverse effect of radiotherapy for head and neck cancers, and is marked by irreversible damage and fibrosis of esophageal muscle tissue. Although mesenchymal stem cell (MSC) therapy is emerging as a promising approach for tissue regeneration, clinical translation remains challenging due to issues with cell viability and differentiation in vivo. This study evaluates the regenerative efficacy of exosomes derived from MSCs transfected with myogenic genes (MyoD, Myogenin, Myf6, referred to as Myo-MIX) using a murine model of radiation-induced esophageal fibrosis. METHODS: Human adipose-derived MSCs were transfected with Myo-MIX plasmids by electroporation, and exosomes were collected from conditioned media using ExoQuick. Nanoparticle tracking analysis and transmission electron microscopy were employed to characterize exosomal size and morphology. A mouse model of localized radiation-induced esophageal injury (10 Gy × 2 fractions) was generated and followed by intramuscular administration of Myo-MIX exosomes. Regenerative and anti-fibrotic outcomes were examined through Masson's trichrome staining, immunohistochemistry (α-SMA, Calponin, CD68), and quantitative RT-PCR. RESULTS: Treatment with Myo-MIX exosomes resulted in a pronounced decrease in fibrosis and inflammatory response compared to PBS-treated controls and naïve MSC-exosome groups. Enhanced restoration of muscular architecture was observed, accompanied by elevated expression of Calponin and α-SMA, and a reduction in CD68 + macrophage infiltration. Gene expression profiling indicated increased levels of myogenic and anti-fibrotic markers in the Myo-MIX exosome-treated group. CONCLUSION: Exosomes from myogenic gene-transfected MSCs significantly enhance esophageal muscle regeneration and attenuate fibrosis after radiation-induced damage. This cell-free therapeutic approach holds potential as a novel and practical strategy for addressing radiation esophagitis in patients receiving radiotherapy for head and neck malignancies.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Regenerative effects of myogenic gene transfected MSC derived exosomes on radiation esophagitis
- Date Crossref
- 05/04/2026
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Seoul National University Hospital Department of Otorhinolaryngology-Head and Neck Surgery pays non établi dans la noticeÉtablissement de santé
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Veterans Health Service Medical Center pays non établi dans la noticeÉtablissement de santé
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Korea University Institute of Chemical Engineering Convergence System pays non établi dans la noticeUniversité ou école supérieure
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Seoul National University Department of Otorhinolaryngology-Head and Neck Surgery pays non établi dans la noticeUniversité ou école supérieure
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National University College pays non établi dans la noticeUniversité ou école supérieure
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Department of Otorhinolaryngology-Head and Neck Surgery pays non établi dans la noticeOrganisation à but non lucratif
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School of Mechanical Engineering pays non établi dans la noticeUniversité ou école supérieure
Department of Otorhinolaryngology-Head and Neck Surgery — Seoul National University Hospital, Veterans Health Service Medical Center et Institute of Chemical Engineering Convergence System — Korea University, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.