Polymer Matrix‐Based 3D Culture Significantly Enhances the Differentiation and Immunomodulatory Functions of Human Adipose‐Derived Stem Cells

Le résumé fourni par la source

Human adipose-derived stem cells (hADSCs) possess immunomodulatory and multipotent properties, making them attractive candidates for regenerative therapies. However, conventional tissue culture plate (TCP)-based 2D adherent cultures or widely used ultra-low attachment (ULA) spheroid systems often compromise differentiation potential and therapeutic efficacy of hADSCs. Here, we report a synthetic polymer matrix, poly-Z, that enables the formation of hADSC spheroids with significantly enhanced biological functions. Compared to ULA or TCP culture systems, poly-Z-cultured hADSC spheroids exhibited improved cell viability, enriched extracellular matrix deposition, elevated pluripotency marker expression, and superior tri-lineage differentiation capacity. Notably, poly-Z spheroids displayed a distinct immunomodulatory signature characterized by significantly elevated secretion of indoleamine-pyrrole 2,3-dioxygenase (IDO) and prostaglandin E2 (PGE2), and showed enhanced in vivo persistence after transplantation. In mouse models of acute colitis and acetaminophen-induced liver injury, poly-Z-cultured hADSC spheroids markedly alleviated inflammation, reduced tissue damage, and modulated pro- and anti-inflammatory cytokine levels more effectively than ULA or TCP-derived cells. These findings establish the poly-Z matrix as a potent 3D culture platform that enhances the functional and therapeutic potential of hADSCs for clinical applications.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.