The regulation of pedicle initiation by androgens in sika deer (Cervus nippon)
Rattachement africain : cn, jp, us, pk. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Background Deer antlers have the potential to contribute to a range of novel biomedical models. Although androgen is undoubtedly an undisputed prerequisite for pedicle initiation (initial stage of antler formation), the molecular mechanisms underlying androgen stimulation remain unclear. Methods Four juvenile male deer (Cervus nippon) were selected: one intact individual and three castrated ones. The castrates were further divided to receive either testosterone or vehicle injections. The antlerogenic periosteum (AP) tissues overlying each frontal crest were collected at successive time points for subsequent analyses. Proteomic analysis was performed using two-dimensional difference gel electrophoresis (2D-DIGE), while cell proliferation and differentiation were assessed through ex vivo tissue explant cultures, in vitro osteoblast induction, and transcriptomic analyses. Results Exogenous androgen induced pedicle initiation. Among the differentially-expressed proteins (DEPs) identified, ACTB, VIM, p53, RNASEL, and CALR were notable for their direct interactions with the androgen receptor (AR). Pathways related to the cell cycle, gene expression, protein metabolism, and signal transduction were found to play key roles in activating pedicle initiation. In serum-free medium, testosterone promoted the proliferation of AP cells in ex vivo tissue explant cultures; however, it showed no effect on osteogenic differentiation of these cells. Conclusion Our findings indicate that testosterone serves as the key factor triggering pedicle initiation. However, this effect likely occurs through the promotion of AP cell proliferation rather than their osteogenic differentiation. Furthermore, the results suggest that CALR may play a pivotal role in regulating the transcription of AR downstream genes during pedicle initiation. These results provide new insights into androgen-regulated mechanisms in deer pedicle initiation.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- The regulation of pedicle initiation by androgens in sika deer (Cervus nippon)
- Date Crossref
- 31/03/2026
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Qingdao Agricultural University pays non établi dans la noticeUniversité ou école supérieure
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National Livestock Breeding Center pays non établi dans la noticeOrganisme public
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Changchun University pays non établi dans la noticeUniversité ou école supérieure
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Chinese Academy of Agricultural Sciences pays non établi dans la noticeOrganisme public
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King University Department of Pharmacy pays non établi dans la noticeUniversité ou école supérieure
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Peking University pays non établi dans la noticeUniversité ou école supérieure
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Islamia University of Bahawalpur pays non établi dans la noticeUniversité ou école supérieure
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University of Veterinary and Animal Sciences pays non établi dans la noticeUniversité ou école supérieure
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Chifeng University pays non établi dans la noticeUniversité ou école supérieure
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College of Animal Science and Technology pays non établi dans la noticeUniversité ou école supérieure
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Shandong Engineering Research Center of Livestock Products Functional Utilization pays non établi dans la noticeStructure de recherche
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Changchun Sci-Tech University Institute of Antler Science and Product Technology pays non établi dans la noticeUniversité ou école supérieure
Qingdao Agricultural University, National Livestock Breeding Center et Changchun University, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.