Detection and Expression of blaKPC-2 and fimH Genes in Klebsiella pneumoniae Clinical Isolates
Rattachement africain : np, us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Klebsiella pneumoniae cause several serious infections, pneumonia, urinary tract infections and bloodstream infections, especially in a immune-compromised patient. There has been rise in antibiotics resistant K. pneumoniae in recent years. This study aimed detection and expression of blaKPC-2 and fimH genes in carbapenemase producing K. pneumoniae that can form biofilms in clinical samples. A total of 608 clinical samples were processed for K. pneumoniae using standard microbiological procedure. Antibiotic resistance was checked using the modified Kirby-Bauer disc diffusion method, and carbapenemase production was tested using a modified carbapenem inactivation method in conjunction with EDTA modified carbapenem inactivation method. In order to detect the presence and expression of resistant genes, bacterial DNA and RNA were extracted. A total of 30 isolates were positive for K. pneumoniae among which 21 (70%) were multi-drug resistant and 18 (85.71%) were carbapenemase producers in MDR isolates. A total of 14 isolates (66.67%) produced biofilm, of which 6 (28.57%) were strong, 6 (28.57%) were moderate and 2 (9.52%) were weak. Both blaKPC-2 and fimH genes were detected in 19 isolates (90.47%) and were actively expressed in 16 (76.19%). These findings show the blaKPC-2 and fimH genes are common in clinical isolates and are actively being expressed. In conclusion, there is urgent need to control highly resistant K. pneumoniae.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Detection and Expression of blaKPC-2 and fimH Genes in Klebsiella pneumoniae Clinical Isolates
- Date Crossref
- 25/03/2026
- Éditeur
- Nepal Journals Online (JOL)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Annapurna Neurological Institute and Allied Sciences pays non établi dans la noticeStructure de recherche
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Hodges University pays non établi dans la noticeUniversité ou école supérieure
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Tribhuvan University Central Department of Microbiology pays non établi dans la noticeUniversité ou école supérieure
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Annapurna Research Center pays non établi dans la noticeStructure de recherche
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SANN International College pays non établi dans la noticeUniversité ou école supérieure
Annapurna Neurological Institute and Allied Sciences, Hodges University et Central Department of Microbiology — Tribhuvan University, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.