ElectroELISAProbe: Low-cost and miniaturized cell for on-site electrochemical microtiter plate readout

Résumé fourni par la source

ABSTRACT The development of portable devices for bioassay readouts has revolutionized point-of-care diagnostics by enabling rapid and decentralized testing of biomarkers, pathogens, and contaminants. Despite the sensitivity of traditional methods like ELISAs, their reliance on bulky and costly optical plate readers limits their applicability in resource-constrained settings. To address these challenges, we have developed the ElectroELISAProbe, a novel electrochemical readout system designed for microtiter plate-based assays. This portable, cost-effective, and user-friendly platform combines mass‑manufactured, ultra‑low‑cost metallic pins as electrodes and simple electronics to achieve robust and adaptable performance across various assays. The ElectroELISAProbe comprises a three-electrode cell housed in a 3D-printed holder, optimized for compatibility with standard microtiter plates. The device was characterized in terms of electrochemical features, demonstrating excellent analytical performance, with high reproducibility (RSDs < 7%). The validation was conducted for detecting Epstein-Barr Virus (EBV) anti-VCA IgG in spiked serum and the matrix metalloproteinase-9 (MMP9) in saliva samples, achieving high recovery rates. Its decentralization potential was endorsed using a portable potentiostat paired with a smartphone interface, enabling field-based applications without performance losses. Furthermore, the versatility of the developed probe supports multiplexing and adaptation to other bioassays requiring electrochemical readouts. These features highlight the ElectroELISAProbe as a promising tool for enhancing accessibility and efficiency in diagnostics, bridging gaps in healthcare, biomedical research and environmental analysis.

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