HP1β and H3K9me3 Regulate Olfactory Receptor Choice and Transcriptional Identity

Le résumé fourni par la source

Diverse epigenetic regulatory mechanisms ensure and modulate cellular diversity. The histone 3 lysine 9 me3 (H3K9me3) post-translational modification participates in silencing lineage-inappropriate genes by restricting access of transcription factors and other regulatory proteins to genes that control cell fate. Mouse olfactory sensory neurons (OSNs) select one olfactory receptor (OR) gene out of 2600 possibilities. This monoallelic and stochastic OR choice occurs as OSNs differentiate and undergo dramatic changes in nuclear architecture. OR genes from different chromosomes converge into specialized nuclear bodies and chromatin compartments, as H3K9me3 and chromatin binding proteins including heterochromatin protein 1 (HP1) are incorporated. In this work, we have uncovered an unexpected role for HP1β in OR choice and neuronal identity that cannot be rescued by HP1α in vivo. With the use of a conditional knock-in mouse model, that after CRE expression replaces HP1β with HP1α, we observe changes in H3K9me3 levels and DNA accessibility over OR gene clusters. These changes alter the expression patterns that partition the mouse olfactory epithelium into five OR expression zones, which results in a reduced OR repertoire that leads to a loss of olfactory sensory neuron diversity. We propose that HP1β modulates the competition of OR promoters for enhancers to promote receptor diversity by establishing repression gradients in a zonal fashion.

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