Quantitative Analysis of Methanolic and Dichloromethane Extracts of Araucaria heterophylla: Formulation as Radical Scavenging Agents and Assessment of Flavonoid Content and Total Phenolic

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Introduction: Araucaria heterophylla, commonly known as Norfolk Island Pine, is a plant with significant medicinal potential due to its rich phytochemical composition. This study aims to explore its antioxidant properties, given its widespread ornamental use and reported pharmacological activities. Secondary metabolites, a type of bioactive molecule with many functions, are abundant in Araucaria heterophylla. The secondary metabolites flavonoids, phenolic acid, and tannin have been found to have anti-inflammatory, antiviral, antibacterial, antioxidant, and anticancer properties. Objective: The objective of this study is to investigate the antioxidant activity of Araucaria heterophylla leaves through extraction and evaluation methods, shedding light on its medicinal relevance and it was also conducted to analyze the phytochemical and determine the total flavonoid, tannin, and phenolic content of Araucaria heterophylla leaves extract using two different solvents (methanol and dichloromethane) using UV-Vis spectrophotometric. Material and Methods: Araucaria heterophylla leaves were gathered, air-dried, and then extracted with methanol. To measure antioxidant activity, we used the DPPH free radical scavenging test, and to find the total antioxidant capacity, we used the phosphor molybdenum technique. To determine the total content of tannin, phenolic, and flavonoid in Araucaria heterophylla, the methanolic extract was made after the plant was recognized by NHB (ID No: DACB 48435). The total phenolic and tannin content was investigated using the Folin-Ciocaltu technique. Result: The methanolic and dichloromethane extracts exhibited significant antioxidant activity, with IC50 values of 45.98 μg/mL and 58.70 μg/mL, respectively. These results suggest the presence of potent antioxidant compounds in Araucaria heterophylla, highlighting its therapeutic potential. The A. heterophylla leaf methanolic and dichloromethane extracts had remarkable antioxidant effects due to their high flavonoid and phenolic content. Conclusion: Araucaria heterophylla leaves demonstrate promising antioxidant properties attributed to their flavonoid and phenolic content. Further research focusing on standardization, stability, and clinical trials could lead to the development of novel therapeutic agents from this plant.

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