Design of a Dual-Detection System for SERRS Cytokine Detection in the NIR Region Simultaneously Matched with Particles and Signal Molecules
Rattachement africain : cn, us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Surface-enhanced resonance Raman scattering (SERRS) represents a synergistic combination of surface-enhanced Raman spectroscopy (SERS) and resonance Raman effects, achieving optimal enhancement when the excitation wavelength, nanoparticle, and molecular resonances are precisely matched. Capitalizing on the superior fluorescence suppression of near-infrared (NIR) excitation is useful for biological applications. We designed a high-performance SERRS tag system through triple-parameter optimization: (1) rationally designed AuAg nanocubes (NCs) with exceptional NIR-SERS activity, (2) resonant Raman reporters (IR1061 and IR808), and (3) 785 nm laser excitation. Multiphysics simulations (COMSOL) revealed theoretical enhancement factors up to 10 7 for AuAg NCs, with experimental SERRS intensities under 785 nm excitation surpassing visible-light performance by at least 1 order of magnitude. This optimized system achieved ultrasensitive detection of clinically relevant cytokines (IL-6 and IL-1β) with detection limits of 1.56 and 6.56 pg/mL, respectively, within 5 min incubation and effectively inhibited antibody-induced nonspecific adsorption in the dual detection, achieving an excellent effect of 15 pg/mL in in actual samples. Therefore, this work not only designed a highly sensitive SERRS biosensor from a principle perspective but also fully utilized the advantages of Raman spectroscopy to truly achieve the dual detection of cytokines, providing a reference for the application of SERRS in clinical detection systems. What is particularly noteworthy is that the combination of AuAg NCs and IR1061 still exhibits excellent detection performance under the 1064 nm excitation light condition, and it is expected to fill the application gap for in vitro diagnostics (IVD) in the NIR-II region.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Design of a Dual-Detection System for SERRS Cytokine Detection in the NIR Region Simultaneously Matched with Particles and Signal Molecules
- Date Crossref
- 16/02/2026
- Éditeur
- American Chemical Society (ACS)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Xiamen University Department of Rheumatology and Clinical Immunology pays non établi dans la noticeUniversité ou école supérieure
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Collaborative Innovation Center of Chemistry for Energy Materials pays non établi dans la noticeStructure de recherche
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China Jiliang University pays non établi dans la noticeUniversité ou école supérieure
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Optica pays non établi dans la noticeOrganisation à but non lucratif
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Tan Kah Kee Innovation Laboratory pays non établi dans la noticeStructure de recherche
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School of Electronic Information pays non établi dans la noticeUniversité ou école supérieure
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Zhangzhou Institute of Technology pays non établi dans la noticeStructure de recherche
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Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM) pays non établi dans la noticeStructure de recherche
Department of Rheumatology and Clinical Immunology — Xiamen University, Collaborative Innovation Center of Chemistry for Energy Materials et China Jiliang University, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.