Additional file 3 of Advancing prion diagnostics: full-length human E200K RT-QuIC substrate facilitates prion detection in tear fluid and improves sensitivity in cerebrospinal fluid

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Supplementary Figure 3: RT-QuIC assays seeded with CSF samples from sCJD, gCJD E200K, and FFI patients were used to compare the seeding conversion efficiencies of hamster–sheep (HS), FL Hu, and FL Hu E200K rec PrP substrates. Positive signal response curves of sCJD samples (n = 43) (A1), gCJD E200K samples (n = 16) (B1), and FFI patient samples (n = 22) (C1) are shown for each rec PrP substrate (chimeric HS, FL Hu, and FL Hu E200K). The area under the curve (AUC) (A2, B2, C2) and the lag phase (A3, B3, C3) were used for quantification. For sCJD and gCJD, the rec PrP FL Hu E200K substrate showed the highest seeding conversion efficiency. No seeding conversion was observed in CSF samples from control subjects (n = 44) (D).

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