Achieving 24-hour reporting turnaround for clinical surveillance detection of eight carbapenemase genes using combined multiplex molecular assays

Résumé fourni par la source

Carbapenemase-producing organisms, including carbapenem-resistant Enterobacterales, Acinetobacter and Pseudomonas species, contribute to over 49,000 infections and more than 4,000 deaths annually in the United States. The Centers for Disease Control and Prevention recommends surveillance using dual swab testing. While a culture-first approach is routinely utilized for carbapenemase-producing organism screening, results require 3 workdays or more. In contrast, Wadsworth Center employs a molecular-first algorithm, combining the Cepheid Xpert Carba-R assay with laboratory developed test multiplex PCR. Using shared reagents for efficiency and specimen preservation, this method detects the most prevalent carbapenemase genes, bla KPC , bla NDM , bla VIM , bla OXA-48-like , and bla IMP , as well as Acinetobacter baumannii -associated genes, bla OXA-23-like , bla OXA-24/40-like , and bla OXA-58-like , and produces actionable results in less than one workday. Wadsworth Center’s rapid carbapenemase-producing organism colonization screening algorithm supports prompt healthcare protocol initiation and epidemiological response.

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